|
KMID : 0363320200410040668
|
|
Journal of Korean Oriental Internal Medicine
2020 Volume.41 No. 4 p.668 ~ p.675
|
|
|
The Effect of Galkunhwanglyeonhwanggum-tang (GGT) and Sopunghwalhyeol-tang (SPT) on Gene Expression Levels of MCP-1, ICAM-1, VCAM-1, and eNOS in HUVECs
|
|
Jeong Hyun-Jin
Jeon Sang-Yoon
Jang Hye-Yeon
Kim Min-Wook
|
|
|
Abstract
|
|
|
Objectives: The aim of this study was to compare the effects of Galkunhwanglyeonhwanggum-tang (GGT), and Sopunghwalhyeol-tang (SPT) on gene expression of MCP-1, ICAM-1, VCAM-1, and eNOS in human umbilical vein endothelial cells (HUVECs).
Methods: HUVECs were treated with GGT and SPT at concentrations of 50, 100, and 200 μg/mL. Gene expression of MCP-1, ICAM-1 ,VCAM-1, and eNOS in HUVECs was analyzed by the polymerase chain reaction (PCR), and electrophoresis was performed to verify the gene expression level.
Results: 1. MCP-1 gene expression was more strongly decreased by SPT than by GGT. 2. ICAM-1 and VCAM-1 gene expressions were more strongly decreased by SPT than by GGT 3. GGT significantly increased eNOS gene expression, but SPT did not.
Conclusions: These findings suggest that GGT and SPT regulate gene expression related to anti-inflammatory effects in HUVECs. Clinical application of these Korean medicines to diseases related to dyslipidemia, such as cardiovascular disease, will require additional in vivo experiments to verify the anti-inflammatory effects of GGT and SPT.
|
|
|
KEYWORD
|
|
|
Galkunhwanglyeonhwanggum-tang, Sopunghwalhyeol-tang, anti-inflammatory effect, HUVEC
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|